You JH, Kang P, Min SS, Seol GH
Journal of Cardiovascular Pharmacology, 2013
Abstract: In this study, we compared the effect of the essential oil of Citrus bergamia Risso [bergamot, bergamot essential oil (BEO)] on the intracellular Ca2+ levels in vascular endothelial (EA) and mouse vascular smooth muscle (MOVAS) cells, using the fura-2 fluorescence technique. BEO caused an initial transient increase in intracellular Ca2+concentration ([Ca2+]i) in EA cells, followed by a decrease, whereas it induced a sustained increase in [Ca2+]i in MOVAS cells. Linalyl acetate (LA) as a major component of BEO-induced [Ca2+]i mobilization was similar to BEO in EA cells. The increase of [Ca2+]i by LA was higher in EA cells than in MOVAS cells. [Ca2+]i rise induced by extracellular Ca2+application was significantly blocked by BEO or LA in EA cells but not in MOVAS cells, suggesting that BEO and LA block Ca2+ influx in EA cells. The present results suggest that BEO and LA differentially modulate intracellular Ca2+ levels in vascular endothelial and smooth muscle cells. In addition, blockade of Ca2+ influx by BEO and LA in EA cells may explain the protective effects of BEO on endothelial dysfunction associated with cardiovascular disease.
You JH, Kang P, Min SS, Et Al. Bergamot essential oil differentially modulates intracellular Ca2+ levels in vascular endothelial and smooth muscle cells: a new finding seen with fura-2. J Cardiovasc Pharmacol. 2013;61(4):324-328.