Bioassay of Herbal Mosquito Repellent Formulated from the Essential Oil of Plants

Lawal HO, Adewuyi GO, Fawehinmi AB, Adeogun AO, Etatuvie SO
Journal of Natural Products, 2012


Malaria, Dengue fever and Filariasis, are serious public health problem in tropical
regions, especially in Africa and Asia and are transmitted through mosquito bites. For
effective control of these diseases, therefore, there is need to prevent individual from
mosquito bites. The effective and safe method is the use of mosquito repellent
naturally obtained from medicinal plants instead of commonly available synthetic
insecticides and repellents such as Organo Phosphorus Carbamate, N, N-Diethyl-3-
methylbenzamide (DEET), and Dichloro Diphenyl Trichloroethane (DDT) which are
carcinogenic and non eco-friendly. In this study, the repellency activities of four
formulated herbal mosquito repellents from the essential oils of some selected MAPPs
of Nigeria were evaluated. The essential oils were extracted by hyrodistillation
method at 50°C using all glass Clevenger apparatus. These were then stored at 4o
c in the refrigerator pending further studies.Graded concentrations (6%, 8%, 10% and
12%) of the essential oils in a complex solution of polyethylene glycol, ethanol and
water were prepared and their mosquito repellency activities was carried out in the
laboratory against Standard Kisumu Strains of Anopheles gambiae. The result showed
that both 8% and 10% formulations have the most promising activity exhibiting 68-
95% repellency activities lasting for 2hours. The standard (odomos®
mosquito repellent cream) exhibited 75-100% repellency activities which also lasted for 2hours
as compared to 8% and 10% formulations. The present study demonstrates the
potential for using essential oils from medicinal plants as mosquito repellent.


Lawal HO, Adewuyi GO, Fawehinmi AB, et al. Bioassay of herbal mosquito repellent formulated from the essential oil of plants.  J Nat Prod. 2012;5:109-155.

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