Vazirzadeh A, Dehghan F, Kazemeini R
Fish and Shellfish Immunology, 2017
An 8- week feeding trial was conducted to investigate the effects of Ducrosia anethifolia essential oil on growth, blood immune parameters and immune related genes expression in juvenile rainbow trout (Oncorhynchus mykiss). Fish were allocated into 4 groups and fed on diet containing different levels of essential oil (0, 0.001, 0.01 and 0.1%) to apparent satiation in 30 min 3 times daily. Growth and immunological parameters were measured every ten days and tissue samples were taken from kidney and spleen on days 10, 30 and 50 to study the expression of IL-1β and TNF-α. The changes of measured parameters in different treatments and over sampling time series were statistically analysis based on repeated measurement method (P < 0.05). Results showed that growth did not affected by essential oil at different treatments. The highest level of hematocrit was observed in 0.001 treatment. The mean of RBC showed no significant differences among treatments. The mean of WBC in 0.01 and 0.1 groups were higher than those in 0.001 and control groups. Total protein, albumin and globulin and serum bactericidal activity showed no significant difference in different treatments. Neither treatments nor sampling times affected serum lysozyme activity. The highest mean of respiratory burst activity was observed in 0.01 group. The highest expression of both IL-1β and TNF-α genes in kidney was observed at 0.001 dose on day 30, while in spleen, the highest expression of IL-1β and TNF-α was obtained on day 30 at doses 0.1 and 0.01%, respectively. In conclusion the results of this study showed that feeding with lower and medium level of D. anethifolia for 30 days led to immunostimulatory effects in juvenile rainbow trout.
Vazirzadeh A, Dehghan F, Kazemeini R. Fish Shellfish Immunol. Changes in growth, blood immune parameters and expression of immune related genes in rainbow trout (Oncorhynchus mykiss) in response to diet supplemented with Ducrosia anethifolia essential oil. 2017 Aug 19;69:164-172. doi: 10.1016/j.fsi.2017.08.022.