Nam SY, Chang MH, Do JS, Seo HJ, Oh HK
Immunopharmacology and Immunotoxicology, 2008
In vivo immunomodulatory effect of essential oil of niaouli (EON) was investigated using a mouse model, in which mice were immunized with keyhole limpet hemocyanin (KLH) and intraperitoneally given EON (less than 500 μl kg-1 body weight). In vivo efficacy of EON for immune potentiation was convinced by significantly higher expression of an activation marker, CD25, on freshly isolated draining lymph node (LN) T cells, but not B cells. However, immunofluoresence analysis failed to show any proportional change in T/B and CD4+/CD8+ T cell ratios. Data of KLH-specific immunoglobulin serum levels showed that EON does not affect humoral immune response. Instead, proliferative response and IFNγ production of LN T cells ex vivo stimulated with KLH were significantly higher in EON-treated group, but not IL-2 and IL-4 production. These results clearly show that EON preferentially upregulates T-cell mediated cellular immunity. We further clarified the accessory cells’ contribution to the EON-mediated potentiation of cellular immunity and found considerably higher production of and TNF-α and IL-12 by splenic macrophages from EON-treated mice when stimulated with lipopolysaccharide (LPS) and IFNγ. Collectively, in vivo EON treatment potentiates T cell-mediated cellular immunity and macrophage activity, but not humoral immunity. The current study provides a rationale for clinical application of EON to control infectious diseases, in particular, those caused by intracellular pathogens.
Nam, SY, Chang MH, Do JS, et al. Essential oil of niaouli preferentially potentiates antigen-specific cellular immunity and cytokine production by macrophages. Immunopharmacol Immunotocxicol. 2008;30(3):459-474.