H2O2-Induced Oxidative Stress, AChE inhibition and mediated brain injury attenuated by Thymus algeriensis

Fatma G, Houda B, Ahmed L
Applied Physiology, Nutrition, and Metabolism, 2018


The aim of the present study is to evaluate the acetylcholinesterase (AChE) inhibition, the antioxidant enzyme activities and malondialdehyde (MDA) levels induced by the hydrophobic fractions of Thymus algeriensis (HFTS) growing in Tunisia. The obtained results showed that Hydrogen peroxide (H2O2), an oxidative stress inducer, act by decreasing body weight and brain weight of rats. Moreover, we found higher MDA levels in the group treated with H2O2 (P<0.05) and a significantly lower activity of catalase (CAT), glutathione peroxidase (GPx), glutathione S-transferase (GST), and superoxide dismutase (SOD) levels, as well as a reduction of reduced glutathione (GSH) activity in the brain tissues of the H2O2-treated rats than the control groups (P<0.05), whereas rat receiving HFTS with H2O2 decline MDA levels in the brain. In contrast, HFTS demonstrated neuroprotective effects in rat brain. Overall exposure to HFTS prior to hydrogen peroxide induced marked dose-dependent increase in radical oxygen species (ROS) scavengers (P<0.05) levels was accompanied with statistically significant decrease in MDA levels (P<0.05) in HFTS-exposed groups when compared with the control. Notably, the activity of AChE was affected by exposure to natural compounds; levels were significantly lower in HFTS-treated rats, or the combination of HFTS and low/high dose of H2O2. Furthermore, histopathological analysis showed that brain injuries occurred at high doses of H2O2 administered alone or with low dose of HFTS, whereas, high dose of essential oil (EO) markedly alleviated neurone degeneration. The obtained results suggest that HFTS alleviates neuroinflammation by acting as an AChE inhibitors (AChEIs) and attenuates H2O2-induced brain toxicity.


Fatma G, Houda B, Ahmed L. H2O2-Induced Oxidative Stress, AChE inhibition and mediated brain injury attenuated by Thymus algeriensis. Appl Physiol Nutr Metab. 2018 May 25.

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