Ohara K, Fukuda T, Okada H, Kitao S, Ishida Y, Kato K, Takahashi C, Katayama M, Uchida K, Tominaga M
The Journal of Biological Chemistry, 2014
Transient receptor potential ankyrin 1 (TRPA1) is a calcium-permeable non-selective cation channel, which is activated by various noxious or irritant substances in nature, including spicy compounds. Many TRPA1 chemical activators have been reported, however, only limited information is available regarding the amino acid residues that contribute to the activation by non-electrophilic activators, while activation mechanisms by electrophilic ligands have been well characterized. We used intracellular Ca2+ measurements and whole-cell patch-clamp recordings to show that eudesmol, an oxygenated sesquiterpene present at high concentrations in the essential oil of hop cultivar Haratau Hersbrucker, could activate human TRPA1. Gradual activation of inward currents with outward rectification by eudesmol was observed in human embryonic kidney-derived 293 cells expressing human TRPA1. This activation was completely blocked by a TRPA1-specific inhibitor, HC03-0031. We identified three critical amino acid residues in human TRPA1 in putative transmembrane domains 3, 4, and 5, namely serine at 813, tyrosine at 840, and serine at 873, for activation by β-eudesmol in a systematic mutational study. Our results revealed a new TRPA1 activator in hop essential oil, and provides a novel insight into mechanisms of human TRPA1 activation by non-electrophilic chemicals.
Ohara K, Fukuda T, Okada H, et al. Identification of Significant Amino Acids in Multiple Transmembrane Domains of Human Transient Receptor Potential Ankyrin 1 (TRPA1) for Activation by Eudesmol, an Oxygenized Sesquiterpene in Hop Essential Oil. J Biol Chem. 2014. pii: jbc.M114.600932.