Pereira NLF, Aquino PEA, Júnior JGAS, Cristo JS, Vieira Filho MA, Moura FF, Ferreira NMN, Silva MKN, Nascimento EM, Correia FMA, Cunha FAB, Boligon AA, Coutinho HDM, Matias EFF, Guedes MIF
Microbial Pathogenesis, 2017
Due to the great biodiversity of its flora, Brazil provides combat tools against bacterial resistance with the utilization of natural products with vegetable origin. Therefore, the present study had as its objective to evaluate the antibacterial potential of the Eugenia uniflora essential oil (EuEO) in vitro, as well as to analyze the modulatory effect of the oil against antibiotics by gaseous contact and to compare them when associated with a LED apparatus. The chemical components were characterised by gas chromatography which revealed the presence of the isoflurane-germacrene, considered the major component (61.69%). The MIC obtained from the EuEO was ≥256 μg/mL for S. aureus and ≥1024 μg/mL for E. coli. When combined with antibiotics, the EuEO presented synergism reducing the MIC when associated, with the exception of gentamicin against E. coli, where an antagonistic effect was observed. The was an interference of the EuEO over the activity of ciprofloxacin when associated with red and blue LED lights, increasing the inhibition halos against S. aureus and E. coli. Norfloxacin presented similar results to ciprofloxacin against S. aureus bacteria. When combined, norfloxacin and the EuEO presented synergism against S. aureus, which did not occur in the combination with ciprofloxacin. Interference occurred only with blue light for E. coli. Thus, it was observed that the EuEO causes changes in the activity of antibiotics, the same occurring with the use of LED lights, without significant differences in the association of the oil and the lights with the antibiotics tested. Further research is needed to elucidate the modulatory effects of the EuEO, as well as its association with LED lights.
Pereira NLF, Aquino PEA, Júnior JGAS et al. In vitro evaluation of the antibacterial potential and modification of antibiotic activity of the Eugenia uniflora L. essential oil in association with led lights. Microb Pathog. 2017 Jul 28. pii: S0882-4010(17)30475-8. doi: 10.1016/j.micpath.2017.07.048.