Uemura T, Yashiro T, Oda R, Shioya N, Nakajima T, Hachisu M, Kobayashi S, Nishiyama C, Arimura GI
Journal of Agricultural and Food Chemistry, 2018
Monoterpenoid perillaldehyde (PA) is the major component in Perilla frutescens leaf essential oil, but its function regarding anti-inflammatory effect is unclear. We explored the anti-inflammatory activity of PA in a dextran sulfate sodium (DSS)-induced colitis mouse model using relief of bodyweight loss (avg. 49.2% mitigation; P = 0.094) and colon damage (avg. 35.3% mitigation; P < 0.05) by administration of PA at a 100 mg/kg dosage. The PA administration resulted in suppression of DSS-induced expression of pro-inflammatory cytokine genes and matrix metalloproteinase-9 in the colon (e.g., avg. 60.6% mitigation for TNF-α mRNA levels; P < 0.05). These effects were confirmed in macrophage RAW264.7 cells stimulated with lipopolysaccharide (LPS). Application of PA induced cell suppression of LPS-induced expressions of genes and proteins of pro-inflammatory cytokines and induced activation of c-Jun N-terminal kinases (JNKs, p54 and p46; P < 0.05) but not nuclear factor-κB p65. The half maximal inhibitory concentration for decreased expression levels of TNF-α mRNA was 171.7 μM. We discuss the in vivo function of PA in amelioration of intestinal inflammation via JNK-mediated cytokine regulation.
Uemura T, Yashiro T, Oda R, et al. Intestinal Anti-Inflammatory Activity of Perillaldehyde. J Agric Food Chem. 2018 Mar 20.