Menezes PP, Araújo AA, Dória GA, Quintans-Júnior LJ, Oliveira MG, Santos MR, Oliveira JF, Matos JR, Carvalho FM, Alves PB, Matos IL, Santos DA, Marreto RN, Silva GF, Serafini MR
Current Pharmaceutical Biotechnology, 2014
The formation of inclusion complexes of Hyptis pectinata essential oil (EOHP), with potent activities such as anti-nociceptive, anti-inflammatory, among others, with β-cyclodextrin (β-CD), was performed using slurry (SC) and paste procedures (PC). The gas chromatography coupled to the mass spectrometry (GC/MS) analysis showed a total volatile content of 36.4% monoterpenes and 63.6% sesquiterpenes in the EOHP. The major components of EOHP were identified as (E)-caryophyllene (54.07%). The characterization of the complex involved the analysis of EOHP, the surface and the total extracted oils. GC/MS results showed significant differences between the original oil, the SC and PC. Thermal characterization indicates the occurrence of complexation, mainly in SC, which presents a thermogravimetry/derivative thermogravimetry (TG/DTG) curve peak at 140-270 ºC, probably related to oil loss. Scanning electron microscopy (SEM) images showed decreased size of the samples especially in the SC product. Additionally, EOHP/ β-CD improves pharmacological profile of EOHP alone in formalin-induced pain protocol in mice.
Menezes PP, Araújo AA, Dória GA, et al. Physicochemical Characterization and Analgesic Effect of Inclusion Complexes of Essential Oil from Hyptis. Curr Pharm Biotechnol. 2014. [Epub ahead of print]