Fazly Bazzaz BS, Khameneh B, Namazi N, Iranshahi M, Davoodi D, Golmohammadzadeh S
Letters in Applied Microbiology, 2018
In the present study, solid lipid nanoparticles containing Eugenia caryophyllata essential oil (SLN-EO) were prepared by high-shear homogenization and ultrasound methods, and used to eradicate pathogens. SLN formulations were evaluated for their size, zeta potential and encapsulation efficacy. The morphological and thermal properties of the formulations were analyzed by TEM and DSC methods. The lead formulations were chosen and tested with MIC, MBC and Time-Kill methods to investigate the antimicrobial activity against Salmonella typhi, Pseudomonas aeruginosa, Staphylococcus aureus and Candida albicans. The particle size of 3 final formulations were 397 ±10.1 nm, 786.9 ± 11 nm and 506.4 ± 22 nm, respectively. The zeta potential of all formulations was negative values. The size of the formulations was slightly increased during 3 months storage at 25 °C. The TEM imaging showed that formulation had spherical shape. The encapsulation efficacy of essential oil was estimated approximately 70%. MIC and MCC values of SLN-EO were lower than those of the oil alone. The time-kill studies showed that SLN-EO was either equivalent to or better than essential oil (P-value < 0.05). The results of this study highlighted the effectiveness of SLN formulations against human pathogens. This article is protected by copyright. All rights reserved.
Fazly Bazzaz BS, Khameneh B, Namazi N, et al. Solid Lipid Nanoparticles (SLN) carrying Eugenia caryophyllata essential oil: The novel nanoparticulate systems with broad-spectrum antimicrobial activity. Lett Appl Microbiol. 2018 Mar 22.