Synergistic antibacterial and antibiofilm efficacy of Nisin in combination with p-Coumaric acid against foodborne bacteria Bacillus cereus and Salmonella typhimurium

Bag A, Chattopadhyay RR
Letters in Applied Microbiology


ABSTRACT:

The aim of the study was to evaluate possible antibacterial and antibiofilm efficacy of a bacteriocin, nisin with two essential oil components linalool and p-coumaric acid in combination against foodborne bacteria Bacillus cereus and Salmonella typhimurium. Their inhibition effects on planktonic cells and pre-formed biofilms were evaluated using microbroth dilution and checkerboard titration methods. Nisin/ p-coumaric acid combination showed synergistic effects against planktonic cells of both the studied bacteria whereas nisin/ linalool combination showed synergistic activity against B. cereus and additive effect against S. typhimurium. In pre-formed biofilms, nisin by itself failed to show >50% antibiofilm efficacy against both the studied bacteria, but in combination with linalool and p-coumaric acid, it exerted > 50% antibiofilm efficacy. On the basis of FICI values, nisin/ p-coumaric acid combination exhibited synergistic antibiofilm activity whereas nisin/linalool combination showed additive effects against pre-formed biofilms of studied bacteria. The results provide evidence that p-coumaric acid due to its synergistic interactions with nisin against planktonic cells and biofilms of both Gram-positive and Gram-negative foodborne bacteria enhanced the antibacterial spectrum of nisin which subsequently may facilitate their use in the food industry. This article is protected by copyright. All rights reserved.

CITATION:

Bag A, Chattopadhyay RR. Synergistic antibacterial and antibiofilm efficacy of Nisin in combination with p-Coumaric acid against foodborne bacteria Bacillus cereus and Salmonella typhimurium. Lett Appl Microbiol. 2017 Aug 16. doi: 10.1111/lam.12793. 


 
[maxbutton id=”2360″]