Bag A, Chattopadhyay RR
Letters in Applied Microbiology
The aim of the study was to evaluate possible antibacterial and antibiofilm efficacy of a bacteriocin, nisin with two essential oil components linalool and p-coumaric acid in combination against foodborne bacteria Bacillus cereus and Salmonella typhimurium. Their inhibition effects on planktonic cells and pre-formed biofilms were evaluated using microbroth dilution and checkerboard titration methods. Nisin/ p-coumaric acid combination showed synergistic effects against planktonic cells of both the studied bacteria whereas nisin/ linalool combination showed synergistic activity against B. cereus and additive effect against S. typhimurium. In pre-formed biofilms, nisin by itself failed to show >50% antibiofilm efficacy against both the studied bacteria, but in combination with linalool and p-coumaric acid, it exerted > 50% antibiofilm efficacy. On the basis of FICI values, nisin/ p-coumaric acid combination exhibited synergistic antibiofilm activity whereas nisin/linalool combination showed additive effects against pre-formed biofilms of studied bacteria. The results provide evidence that p-coumaric acid due to its synergistic interactions with nisin against planktonic cells and biofilms of both Gram-positive and Gram-negative foodborne bacteria enhanced the antibacterial spectrum of nisin which subsequently may facilitate their use in the food industry. This article is protected by copyright. All rights reserved.
Bag A, Chattopadhyay RR. Synergistic antibacterial and antibiofilm efficacy of Nisin in combination with p-Coumaric acid against foodborne bacteria Bacillus cereus and Salmonella typhimurium. Lett Appl Microbiol. 2017 Aug 16. doi: 10.1111/lam.12793.