Zhang LL, Ma XL, Wang D, Yu P, Huang LQ, Zhang XL, Dai ZB
Zhongguo Zhong Yao Za Zhi, 2017
ABSTRACT:
Nerolidol is an important constituent of terpenoid essential oil and has excellent anti-tumor, anti-bacterial, and anti-oxidative properties. For realizing heterogenous production of nerolidol, our research firstly integrated the codon-optimized Actinidia sinensis nerolidol synthase gene (NES) into the terpenoid chassis strain FPP-001, and obtained NES-001 that could produce 2.71 mg•L⁻¹ nerolidol. Then, the N-terminal of the NES was fused with FPS by linker peptide GGGS. With this strategy, nerolidol production improved by 59.80-fold, reaching 162.07 mg•L⁻¹. Finally, by introduction of auxotrophic marker TRP1 in NES-002, the resulting strain NES-003 could produce 1 711.53 mg•L⁻¹ by high cell density fermentation method. This study provides the basis for the fermentative production of nerolidol and other sesquiterpenoids.
CITATION:
Zhang LL, Ma XL, Wang D et al. Construction of cell factories for high production of nerolidol in Saccharomyces cerevisiae (Chinese). Zhongguo Zhong Yao Za Zhi. 2017 Aug;42(15):2962-2968. doi: 10.19540/j.cnki.cjcmm.20170714.002. Chinese.