Zhang LL, Ma XL, Wang D, Yu P, Huang LQ, Zhang XL, Dai ZB
Zhongguo Zhong Yao Za Zhi, 2017
Nerolidol is an important constituent of terpenoid essential oil and has excellent anti-tumor, anti-bacterial, and anti-oxidative properties. For realizing heterogenous production of nerolidol, our research firstly integrated the codon-optimized Actinidia sinensis nerolidol synthase gene (NES) into the terpenoid chassis strain FPP-001, and obtained NES-001 that could produce 2.71 mg•L⁻¹ nerolidol. Then, the N-terminal of the NES was fused with FPS by linker peptide GGGS. With this strategy, nerolidol production improved by 59.80-fold, reaching 162.07 mg•L⁻¹. Finally, by introduction of auxotrophic marker TRP1 in NES-002, the resulting strain NES-003 could produce 1 711.53 mg•L⁻¹ by high cell density fermentation method. This study provides the basis for the fermentative production of nerolidol and other sesquiterpenoids.
Zhang LL, Ma XL, Wang D et al. Construction of cell factories for high production of nerolidol in Saccharomyces cerevisiae (Chinese). Zhongguo Zhong Yao Za Zhi. 2017 Aug;42(15):2962-2968. doi: 10.19540/j.cnki.cjcmm.20170714.002. Chinese.