Pekmezovic M, Rajkovic K, Barac A, Senerović L, Arsic Arsenijevic V
Biochemical Engineering Journal, 2015
The antifungal effect of essential oils (EOs) of Thymus vulgaris L. (EOT. vulgaris) and Cinnamomum cassia L. (EOC. cassia) against Aspergillus flavus spores was evaluated by determining minimum inhibitory concentration, minimum fungicidal concentrations and fungicidal kinetics. Kinetic model of fungicidal activity of individual EOT. vulgaris and EOC. cassia was developed and its parameters were used to make EO mixture with optimal ratio of individual EOs. Synergism and speed of fungicidal effect of EO mixtures against A. flavus spores were evaluated. Kinetic model revealed optimal ratio EOT. vulgaris: EOC. cassia as 14:1 in mixture. Observed result was validated by comparing fungicidal effect of this mixture to commonly used ratio 1:1 and intermediate 7:1 EO mixtures. All three mixtures showed partial fungicidal synergism, but the time point of fungicidal effect was different. The fastest fungicidal effect was observed in 14:1 EO mixture (after 90min), followed by 7:1 (110min) and 1:1 (180min). The difference in the speed of fungicidal effect could not be detected using standard microdilution susceptibility tests, which demonstrated the importance and usefulness of developed kinetic model for further investigations.
Pekmezovic M, Rajkovic K, Barac A, Senerović L, Arsic Arsenijevic V. Development of kinetic model for testing antifungal effect of Thymus vulgaris L. and Cinnamomum cassia L. essential oils on Aspergillus flavus spores and application for optimization of synergistic effect. Biochem Eng J. 2015;99:131-137.